Introduction
Configuration
Upload Trace
Execution
Results
Database
WebTraceMiner Configuration
If you want to use our demo data, please click "Save Configuration" to proceed with default settings. For general users to process their own data, the only required configuration is in the "Adapter and Restriction Enzyme Site" section.
Global Settings
Help
Config Name:
Command Dir:
Debug Mode:
Yes
No
Process Dir:
Trace Type:
EST
Parameter Dir:
Base Calling
Help
Phred:
Staden Package io_lib:
Version:
phred version: 0.020425.c
phred version: 0.000925.c
Parameter:
Nice:
Phd2fasta:
View current Phred parameters
Upload Parameter:
Version:
1.9.0
Quality Trimming
Help
Min Length:
100
75
50
Moving Window:
(size)
Qual Threshold:
Phred Quality
≥
10
Phred Quality
≥
16
Phred Quality
≥
20
Phred Quality
≥
30
Threshold:
13
14
15
16
17
18
(threshold, 13-18)
Vector Screening
Help
Cross_Match
Vmatch
BLAST
Executable:
-minmatch:
10
12
14
20
-minscore:
20
30
-penalty:
-2
-1
Executable:
-l minmatch:
10
12
14
20
(Min Match Length)
-identity:
80
85
90
95
100
-h :
0
1
2
3
4
(Hamming Distance)
Executable:
Parameter:
Vector Folder:
Vector File:
vector
Vector Detect:
1
Upload Vector:
Adapter and Restriction Enzyme Site
Help
Adaptor 1:
Enzyme 1:
GAATTC :: EcoRI
GATATC :: EcoRV
AAGCTT :: HindIII
CATATG :: NdeI
GCGGCCGC :: NotI
GTAC :: RsaI
GTCGAC :: SalI
CCCGGG :: SmaI
CTCGAG :: XhoI
Adaptor 2:
Enzyme 2:
GAATTC :: EcoRI
GATATC :: EcoRV
AAGCTT :: HindIII
CATATG :: NdeI
GCGGCCGC :: NotI
GTAC :: RsaI
GTCGAC :: SalI
CCCGGG :: SmaI
CTCGAG :: XhoI
Error Base:
0
1
2
Error Base:
1
0
Need new adapter or restriction enzyme?
Please send us an
email
.
Poly(A)/(T) Detection
Help
Poly A:
Yes
No
Poly T:
Yes
No
-minlength:
-minlength:
-maxmismatch:
0
1
2
-maxmismatch:
0
1
2
EST Direction
Help
Non-directional:
Directional:
OK
Perl Regular Expression:
3' EST:
5' EST:
Upload EST Direction File:
5' EST:
3' EST:
You must save the configuration before you can proceed with the next step.